First Graduation / A Thousand Emotions / Sadness / Our Old School / Everyone (Japanese: 「初めての卒業」 「万感」 「悲しみ」 「母校」 「みんな」) is the twenty sixth and final episode of Azumanga Daioh which ends here.
Before the graduation ceremony, Osaka reveals to Chiyo that she suffers from hay fever, so she has to keep tissues and eyedrops with her at all times. Later, at the ceremony, Chiyo is presented with the prize of best academic performance, and brims with joy upon receiving a standing ovation started by Tomo and Osaka. When "Aogeba tōtoshi", the song that marks Japanese high school graduation, is sung, Chiyo is unable to hold back her tears upon remembering that she will have to leave her school behind. Later, Yukari comes into class devastated because she lost her wallet and, while handing out the diplomas, everyone starts contributing with money, though at low quantities. She quickly changes her mood when Nyamo comes with her retrieved wallet however. Nyamo is effusively congratulated by her class for all the work, Kimura bursts into tears when mentioning that the students will leave his company, and Yukari receives a large flower bouquet contributed by every student in her class. Afterwards, Kaorin asks Tomo to take a picture of her with Sakaki and demands a copy be sent to her. While Kagura thinks of obtaining a piece of furniture from the class as a keepsake, Yukari explains that she already has her memories of high school to take with her. Chiyo proposes that everyone goes to the amusement park again as a graduation trip, which Yomi accepts to come to this time, but she feels insulted when Tomo says they should wait after her results come out. When leaving school, Chiyo takes her time to bid it farewell with one last bow. On the day that the entrance exam results are posted, Yomi is initially shocked when Tomo informs her that she did not pass, but Yomi rechecks this and sees that she has been approved along with the others. Finally, as they all head for the train station toward the amusement park, Chiyo realizes that, even after graduation, they will always be together.
Once again, photos from the Okinawa trip are shown but this time in Chiyo's room.
Sakaki refers to all her failed attempts to pet Kamineko when she excuses herself for always trying to.
When it's time to sing, Yomi again does a terrible job, irritating those standing close to her (mainly Tomo).
Since Yomi couldn't come the last time the girls went to Magical Land due to being sick, Chiyo comes up with the idea of going there again for their graduation trip. On the day of their trip, Chiyo also suggests for them to ride on a roller coaster they tried the last time again.
Osaka takes the good luck charm Chiyo gave Yomi and attempts to refill it with her brainpower, because Yomi had yet to see whether she would be accepted into college or not.
What Tomo wanted to tell Osaka when she called her after taking Kaorin's and Sakaki's picture.
If Yukari went on another vacation with her former students.
Instead of trying to pet Kamineko when she sees it, Sakaki apologizes for always trying to pet it. This confuses Kamineko, who tries to make it seem like it wants Sakaki to pet it, only to bite her again when she tries to.
Osaka brings tissues and eye drops to school because of her hay fever. She amuses Kagura greatly with her unusual sneezes.
At the graduation ceremony, Chiyo receives an award for being the highest achieving student from the graduating class. A standing ovation started by Tomo and Osaka is the result, moving Chiyo to tears.
Chiyo cries because she is so anxious to leave her classmates. Yukari also cries when she sees her students for the last time, but it turns out this was because she had lost her wallet. Osaka comes up with the idea of donating money to Yukari, but this only makes Yukari angry because she thinks she isn't getting enough.
Kaorin convinces Tomo to take a picture of her and Sakaki together, but Tomo tilts the camera down. Kaorin screams at her, so Tomo agrees to take a new one.
Kagura gets emotional in front of Yukari because she wants to take something back with her to remember her high school days, and Yukari responds that she already has her precious memories to take back with her.
Chiyo bows to the school before she leaves it for the last time.
The girls find out that Yomi has been accepted into college after all and they happily set off to Magical Land to celebrate their graduation. And thus, the series comes to an end.
This will be the first time I've ever graduated!
I'm sorry … for always trying to pet you against your will.
— Sakaki (to Kamineko)
"Female College Graduate Employment Rate At An All Time Low!" it says. You know, at this rate, it might be useless even if you do get in!
— Tomo (to Yomi)
Uh-uh, they're no ordinary tissues. These ones are high class! For real!
— Osaka (to Chiyo-chan)
I got eye drops too! Although, I'm one of those people who can't apply 'em unless I lie down!
Hay fever looks like fun!
Hey! Why is it that I'm not the one representing the graduating class?
— Tomo (to Yomi)
Hey! You're wrecking the mood!!
— Tomo (to Yukari)
I DON'T NEED YOUR CHEAP SYMPATHY!!!
— Yukari (to her class)
Okay, time for final words. Well, goodbye everybody …
… BUT Kaorin and I will be together forever!! [cracks neck]
— Kimura (to his class)
They're wonderful. I WONDER WHO WILL BE FIRST TO WILT AND DIE!!!
— Yukari (on the flowers given to her by her class representing her students)
You guys should be just fine, but no guarantees!
— Yukari (to Chiyo)
NO, YOU DON'T "GOT IT", YOU TILTED THE CAMERA DOWN!!!
— Kaorin (to Tomo) [furiously]
Yukari-sensei, I've been thinkin'. Would it be bad if I took a chair or desk home?
We'll do it before Yomi's school makes a posting. Because it'd be a real DOWNER if she doesn't make it in!!
— Tomo (on the Magical Land trip)
ARGH!!! YOU PISS ME OFF SO MUCH!!!
— Yomi (to Tomo)
So YOU were serious about going to Magical Land immediately after the announcement was made!?
Don't worry, we've already thought of what to say to comfort you! Right?
— Tomo (to Yomi, on the possibility of her failing to get into college)
No!! She's still in the middle of imbuing it with her power! You weren't supposed to … You weren't supposed to look!! [goes to check] Huh? What's the…? It's there.
— Yomi (to Tomo, on her number)
I see! Even when we've graduated … We'll still be together!